Supramolecular Protein Assemblies Based on DNA Templates

DNA plays an important role in the process of protein assembly. DNA viruses such as the M13 virus are typical examples in which single DNA genomes behave as templates to induce the assembly of multiple major coat protein (PVIII) monomers. Thus, the design of protein assemblies based on DNA templates attracts much interest in the construction of supramolecular structures and materials. With the development of DNA nanotechnology, precise 1D and 3D protein nanostructures have been designed and constructed by using DNA templates through DNA-protein interactions, protein-ligand interactions, and protein-adapter interactions. These DNA-templated protein assemblies show great potential in catalysis, medicine, light-responsive systems, drug delivery, and signal transduction. Herein, we review the progress on DNA-based protein nanostructures that possess sophisticated nanometer-sized structures with programmable shapes and stimuli-responsive parameters, and we present their great potential in the design of biomaterials and biodevices in the future.

DNA在蛋白质组装过程中发挥着重要作用。 M13 病毒等 DNA 病毒是典型的例子，其中单个 DNA 基因组作为模板诱导多个主要外壳蛋白 (PVIII) 单体的组装。因此，基于 DNA 模板的蛋白质组装设计引起了人们对超分子结构和材料构建的极大兴趣。随着DNA纳米技术的发展，利用DNA模板，通过DNA-蛋白质相互作用、蛋白质-配体相互作用、蛋白质-接头相互作用，设计和构建了精确的1D和3D蛋白质纳米结构。这些以 DNA 为模板的蛋白质组装体在催化、医学、光响应系统、药物输送和信号转导方面显示出巨大的潜力。在此，我们回顾了基于 DNA 的蛋白质纳米结构的进展，这些结构具有复杂的纳米尺寸结构，具有可编程的形状和刺激响应参数，并展示了它们在未来生物材料和生物设备设计中的巨大潜力。