Simultaneous detection of dual nucleic acids using a SERS-based lateral flow assay biosensor

A new class of surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) biosensor has been developed for the simultaneous detection of dual DNA markers. The LFA strip in this sensor was composed of two test lines and one control line. SERS nano tags labeled with detection DNA probes were used for quantitative evaluation of dual DNA markers with high sensitivity. Target DNA, associated with Kaposi's sarcoma-associated herpesvirus (KSHV) and bacillary angiomatosis (BA), were tested to validate the detection capability of this SERS-based LFA strip. Characteristic peak intensities of SERS nano tags on two test lines were used for quantitative evaluations of KSHV and BA. The limits of detection for KSHV and BA, determined from our SERS-based LFA sensing platform, were estimated to be 0.043 and 0.074 pM, respectively. These values indicate approximately 10 000 times higher sensitivity than previously reported values using the aggregation-based colorimetric method. We believe that this is the first report of simultaneous detection of two different DNA mixtures using a SERS-based LFA platform. This novel detection technique is also a promising multiplex DNA sensing platform for early disease diagnosis.

一种新型基于表面增强拉曼散射 (SERS) 的侧流分析 (LFA) 生物传感器已开发出来，用于同时检测双 DNA 标记。该传感器中的 LFA 条由两条测试线和一条控制线组成。使用标记有检测DNA探针的SERS纳米标签对双DNA标记进行高灵敏度的定量评估。对与卡波西肉瘤相关疱疹病毒 (KSHV) 和杆菌性血管瘤病 (BA) 相关的目标 DNA 进行了测试，以验证这种基于 SERS 的 LFA 试纸条的检测能力。使用两条测试线上的 SERS 纳米标签的特征峰强度对 KSHV 和 BA 进行定量评估。根据我们基于 SERS 的 LFA 传感平台确定的 KSHV 和 BA 的检测限估计分别为 0.043 pM 和 0.074 pM。这些值表明灵敏度比之前使用基于聚合的比色方法报告的值高出大约 10 000 倍。我们相信这是使用基于 SERS 的 LFA 平台同时检测两种不同 DNA 混合物的第一份报告。这种新颖的检测技术也是一种有前景的用于早期疾病诊断的多重 DNA 传感平台。