One-step detection of melamine in milk by hollow gold chip based on surface-enhanced Raman scattering

A hollow gold (HG) chip with high surface-enhanced Raman scattering (SERS) capability was fabricated and used to monitor the adulteration of milk with melamine. This chip was fabricated with self-assembled hollow gold nanospheres (HGNs) on glass wafers through electrostatic interaction. There are two important advantages for the use of this HG chip as a detection platform. First, HGNs show a strong SERS enhancement from individual particles due to their capability to localize the electromagnetic fields around the pinholes in hollow shells. Second, the HG chip improves the limit of detection through the enrichment effect. The characteristic SERS peak of melamine was used to distinguish it from other kinds of proteins or amino acids, and its intensity was used to monitor the percentage of melamine in milk. With its simple detection procedure (no pretreatment or separation steps), decreased processing time and low detection limit, this HG chip shows a strong potential for broad applications in melamine detection from real samples.

制造了具有高表面增强拉曼散射（SERS）能力的空心金（HG）芯片，并用于监测牛奶中三聚氰胺的掺假。该芯片是通过静电相互作用在玻璃晶圆上自组装空心金纳米球（HGN）制成的。使用该HG芯片作为检测平台有两个重要的优点。首先，HGN 由于能够将电磁场定位在空心壳的针孔周围，因此表现出单个粒子的强烈 SERS 增强作用。其次，HG芯片通过富集效应提高了检测限。利用三聚氰胺的特征SERS峰将其与其他种类的蛋白质或氨基酸区分开来，并利用其强度来监测牛奶中三聚氰胺的百分比。凭借其简单的检测程序（无需预处理或分离步骤）、缩短的处理时间和较低的检测限，该 HG 芯片在实际样品的三聚氰胺检测中显示出广泛应用的巨大潜力。