Construction of protein nanowires through Cucurbit[8]uril-based highly specific host-guest interactions: An approach to the assembly of functional proteins

Getting your proteins in a row: Self-assembled protein nanowires were designed by utilizing highly specific supramolecular interactions of cucurbit[8]uril (CB[8]) and a tripeptide FGG-tag attached on the N-termini of dimeric glutathione S-transferase (GST). With the aid of enzyme simulation, model protein GST was converted into a selenoenzyme glutathione peroxidase mimic, and the CB[8]-induced Se-FGG-GST(Y6C) nanowires demonstrated excellent antioxidative capacity.

让蛋白质排列整齐：自组装蛋白质纳米线是利用葫芦[8]尿素 (CB[8]) 和附着在二聚体谷胱甘肽 S-转移酶 (GST) N 末端的三肽 FGG 标签的高度特异性超分子相互作用来设计的。借助酶模拟，模型蛋白GST被转化为硒酶谷胱甘肽过氧化物酶模拟物，CB[8]诱导的Se-FGG-GST(Y6C)纳米线表现出优异的抗氧化能力。